Andreas Natsch1*, Barbara F. Günthardt1, Elise Corbi2, Christophe Pérès2, André Düsterloh3, Hans Leijs4, Michel van Strien4, Ulrika Nilsson5, Michael J. Calandra6, Sabine Leocata6, Matthias Vey7, Cecile Gonzalez7,
Hans J. Bender7, Alain Chaintreau8, Hugues Brévard9, Neil Owen10, Florian Stintzing11, Jean-Marie Aubry12, Anna Börje5, Ann-Therese Karlberg13, David W. Roberts14, Elena Giménez-Arnau15, and Véronique Rataj12
1 Givaudan Schweiz AG, Switzerland; 2 CHANEL, France; 3 DSM Nutritional Products, Switzerland; 4 International Flavors & Fragrances, Netherlands; 5 Stockholm University, Sweden; 6 Firmenich US, USA; 7 IDEA management team, Belgium; 8 Firmenich, Switzerland; 9Robertet, France; 10 Givaudan International, UK; 11 Wala, Germany; 12 University of Lille, France; 13 University of Gothenburg, Sweden; 14 St. Moores University, Liverpool, UK; 15 University of Strasbourg, France.
Recently, a high frequency of positive patch tests to oxidized linalool and limonene was reported from multiple clinical centers. However, there is a lack of data indicating potential sources of consumer exposure to sensitizing doses of terpene hydroperoxides, which are the key sensitizers in preparations of these oxidized materials. To understand the frequent patch tests and define potential actions needed, this gap needs to be closed. Within the IDEA project (International Dialogue for the Evaluation of Allergens), three workshops were organized to look at the clinical data, the conditions leading to hydroperoxide formation and the question of significant exposure to hydroperoxides from fragranced products. IDEA formulated a program that will drive analytical and mechanistic understanding of these potential reactions which eventually will allow the development of a framework, broadly applicable to address the pre-/pro-hapten question in a meaningful approach. As one key action, a hydroperoxide analytical taskforce was established. This group (i) reviewed the analytical challenge, (ii) organized a reliable source for pure analytical standards, and (iii) started two method evaluation studies to compare different analytical methods to detect hydroperoxides in essential oils. LC/MS and GC/MS methods with derivatization were tested. Based on the results of these initial steps, a new study was planned to compare one of several promising methods for reproducibility between laboratories. At the same time, a broad range of different methods were compared on the same products. All these analysis was done on two real-life consumer products (eau de parfum and eau de toilette) spiked with defined, but blinded, levels of hydroperoxides. Full results of this study will be presented to give a perspective on the status of this IDEA guided project and discuss the remaining analytical challenge to truly measure exposure of the public to sensitizing hydroperoxides.
Dr. Ian White, a past Chair of the SCCS, is a member of the Supervisory group, a small group of independent internally recognized experts, responsible for the scientific aspects of the IDEA project. He has been involved in a number of the IDEA Workshops. He will be presenting the progress of the IDEA programme and a vision of the future.
The IDEA project is a multi-stakeholder project designed to address the issue of skin allergies from fragrance ingredients. The objective is to develop a scientifically sound, robust methodology for the risk assessment of fragrances which will ensure a high level of consumer protection.